Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A_dexamethasone (DEX)_60 min
cell line
MCF10A
treated with
dexamethasone (DEX)
treatment time
60 min
chip antibody
rabbit anti-GR polyclonal antibody (Thermo Fisher Scientific, PA1-511A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell fixation and chromatin immunoprecipitation were performed as described (Blecher-Gonen et al., 2013) Library construction and sequencing were performed as described (Blecher-Gonen et al., 2013)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
4650835
Reads aligned (%)
42.1
Duplicates removed (%)
50.4
Number of peaks
1164 (qval < 1E-05)

hg38

Number of total reads
4650835
Reads aligned (%)
43.5
Duplicates removed (%)
49.2
Number of peaks
1163 (qval < 1E-05)

Base call quality data from DBCLS SRA