Antigen

Antigen Class

TFs and others

Antigen

NR3C1

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

MCF10A_dexamethasone (DEX)_20 min

cell line

MCF10A

treated with

dexamethasone (DEX)

treatment time

20 min

chip antibody

rabbit anti-GR polyclonal antibody (Thermo Fisher Scientific, PA1-511A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell fixation and chromatin immunoprecipitation were performed as described (Blecher-Gonen et al., 2013) Library construction and sequencing were performed as described (Blecher-Gonen et al., 2013)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

5201635

Reads aligned (%)

76.8

Duplicates removed (%)

46.2

Number of peaks

578 (qval < 1E-05)

hg19

Number of total reads

5201635

Reads aligned (%)

76.1

Duplicates removed (%)

47.4

Number of peaks

574 (qval < 1E-05)