Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Larvae
Cell type
Imaginal brain disc
NA
NA

Attributes by original data submitter

Sample

source_name
third instar larval imaginal discs and brains
tissue
third instar larval imaginal discs and brains

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total genomic DNA was extracted from CNS and disc materials using XXX. Invitrogen Dynabeads (M-280 sheep anti-rabbit IgG) were used for Chromatin immunoprecipitation in place of protein A or G agarose/salmon sperm DNA beads. ChIP reactions were performed with 1:100 dilutions of anti-Pho (Brown et al., 2003), anti-Spps (Brown and Kassis 2010), anti-Ph (Donna-Arndt-Jovin), 1:200 dilutions of anti-E(z) (rabbit polyclonal raised against bacterially expressed full length E(z)), anti-Psc (Nicole Francis), anti-Pc (Santa Cruz Biotech Inc, sc25762), and anti-H3K27me3 (Millipore, 17-622).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
12112903
Reads aligned (%)
12.8
Duplicates removed (%)
26.0
Number of peaks
460 (qval < 1E-05)

dm3

Number of total reads
12112903
Reads aligned (%)
12.9
Duplicates removed (%)
24.6
Number of peaks
390 (qval < 1E-05)

Base call quality data from DBCLS SRA