GSM2734694: ChIPSeq UP CD4T +IL2 STAT5B; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Stat5b
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD4T STAT5B, +IL2, UP deletion
strain background
C57BL/6
genotype/variation
UP deletion
tissue
Spleen
cell type
pre-activated T cells
treated with
IL-2 (100 U/ml, 2 nM) for 1hr
chip antibody
anti-STAT5B (R&D systems)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, and cross-linked with 1% formaldehyde (methanol-free, Pierce, Rockford, IL) at room temperature for 10 min. After sonication, fragmented chromatin equivalent to 10 million cells was immunoprecipitated with control rabbit IgG (Santa Cruz Biotechnology, Dallas TX) or indicated antibodies and Magna ChIPTM Protein A+G Magnetic Beads (Millipore, Billerica MA). ChIP-Seq DNA libraries were prepared using KAPA LTP Library Preparation Kit (Kapa Biosystems, Wilmington, MA) and indexed primers (BIOO Scientific, Austin, TX), and the libraries were then sequenced. For RNA-Seq, KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Wilmington, MA), and each library was indexed using barcoded primers (BIOO Scientific, Austin, TX). Barcoded PCR products were purified on 2% E-Gel, 250-400 bp fragments were purified, quantified on Qbit (Invitrogen), mixed, and sequenced on an Illumina Genome Analyzer II or HiSeq2000 platform. PCR products were barcoded (indexed) and sequenced on Illumina Genome Analyzer II or HiSeq 2000 platform.