Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

HSC in vitro expanded bone marrow cells transduced with Nup98-HoxB4

cell type

in vitro expanded bone marrow cells transduced with Nup98-HoxB4

strain

B6

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Libraries were prepared according to Illumina's instructions accompanying the Agilent_DNA _Kit _100. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were generated and sequenced on the Genome Analyzer following the Illumina manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

60193739

Reads aligned (%)

82.9

Duplicates removed (%)

42.0

Number of peaks

761 (qval < 1E-05)

mm9

Number of total reads

60193739

Reads aligned (%)

82.7

Duplicates removed (%)

41.9

Number of peaks

846 (qval < 1E-05)