GSM1164228: H3K27ac ChIP-seq for GM19141; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
Lymphoblastoid cell line
Tissue
blood
Lineage
mesoderm
Description
parental cell type to lymphoblastoid cell lines
Attributes by original data submitter
Sample
source_name
lymphoblastoid cell line
biomaterial_provider
GM19141
cell line
GM19141
cell type
lymphoblastoid cell line
population
YRI
ethnicity
Yoruba
country of origin
Nigeria
chip antibody
H3K27ac (Abcam ab4729)
chip antibody manufacturer
Abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted using protocol described in Cain et al. 2011, except that for some samples chromatin was sheared with a Covaris S2 (settings: 40 min duty cycle 20%, intensity 8, 200 cycles/burst, 500 uL at a time in 12 x 24 mm tubes). Prepared Illumina sequencing libraries as described by the ENCODE Project Consortium (2011), starting with 20 uL of ChIP output or 4 ng of pooled input sample.