Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
R1-D567
NA
NA

Attributes by original data submitter

Sample

source_name
R1-D567_AR_SCBT_RT
cell line
R1-D567
cell type
Transcription activator-like effector nuclease genetically-engineered cell lines derived from CWR-R1 PCa cells
sample group
radiated cells
chip antibody
Androgen Receptor (SCBT, sc-816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
17020007
Reads aligned (%)
94.1
Duplicates removed (%)
3.4
Number of peaks
629 (qval < 1E-05)

hg38

Number of total reads
17020007
Reads aligned (%)
96.1
Duplicates removed (%)
2.3
Number of peaks
635 (qval < 1E-05)

Base call quality data from DBCLS SRA