Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
cell type
mouse embryonic stem cells
genotype
Dnmt3a/3b DKO
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. See http://bioinformatics-renlab.ucsd.edu/RenLabLibraryProtocolV1.pdf

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
32960556
Reads aligned (%)
90.5
Duplicates removed (%)
9.3
Number of peaks
768 (qval < 1E-05)

mm9

Number of total reads
32960556
Reads aligned (%)
90.3
Duplicates removed (%)
9.4
Number of peaks
835 (qval < 1E-05)

Base call quality data from DBCLS SRA