Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rbf

Cell type

Cell type Class
Adult
Cell type
Flight muscles
NA
NA

Attributes by original data submitter

Sample

source_name
flight muscles
chip antibody
anti-Rbf (mouse, DX3/DX5, ratio 1:1, Du W. et al, Genes Dev. 1996;10(10):1206-18)
developmental stage
Pharate stage (96h APF at 25 degrees C)
genotype
P{UAS-mCherry-RNAi}attP2 ; P{GAL4-Mef2.R}3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from flight muscles was extracted using homogenizer and fixed in 1.8% formaldehyde. Then lysates were sheared using a Sonifier, and chromatin was immunoprecipitated with antibodies as in Zappia, M. P. and Frolov, M. V. Nat. Commun. 7:10509 (2016) ChIPSeq libraries were generated using an Illumina ChIP-SEQ kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
42959354
Reads aligned (%)
5.6
Duplicates removed (%)
35.3
Number of peaks
1358 (qval < 1E-05)

dm6

Number of total reads
42959354
Reads aligned (%)
5.4
Duplicates removed (%)
37.0
Number of peaks
941 (qval < 1E-05)

Base call quality data from DBCLS SRA