anti-Rbf (mouse, DX3/DX5, ratio 1:1, Du W. et al, Genes Dev. 1996;10(10):1206-18)
developmental stage
Pharate stage (96h APF at 25 degrees C)
genotype
P{UAS-mCherry-RNAi}attP2 ; P{GAL4-Mef2.R}3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from flight muscles was extracted using homogenizer and fixed in 1.8% formaldehyde. Then lysates were sheared using a Sonifier, and chromatin was immunoprecipitated with antibodies as in Zappia, M. P. and Frolov, M. V. Nat. Commun. 7:10509 (2016) ChIPSeq libraries were generated using an Illumina ChIP-SEQ kit