GSM2720353: ATF6+/+ hMSC tunicamycin-1 IgG ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
MSC
cell type
MSC
treatment
tunicamycin
passages
p5
chip antibody
IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted with Trizol reagent. To obtain the genomic DNA fragments of interest, the cells were crosslinked and lysed, and then the chromatin was sheared into fragments using sonication. Then samples were incubated with anti-H3K4me3 antibody(Abcam, ab8580) or anti-FLAG(Sigma, F1804) antibody overnight. The DNA was de-crosslinked and extracted on the next day. 1.5 μg of total RNA was used to construct RNA sequencing libraries by TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s recommended protocol. After the DNA was de-crosslinked and extracted, the ChIP-sequencing library was constructed by TruSeq DNA Sample Preparation Kit (Illumina) or NEBNext® DNA Library Prep Reagent Set (NEB) according to the manufacturer’s instructions.