Antigen

Antigen Class

TFs and others

Antigen

Gabpa

Cell type

Cell type Class

Blood

Cell type

CD4+ T cells

NA

NA

Sample

source_name

CD4 T cells

tissue

CD4 T cells

strain

C57BL/6

antibody

anti-GABPalpha (sc-22810X, Santa Cruz).

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and GABPα-DNA complexes were isolated with anti-GABPα antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

164483236

Reads aligned (%)

69.9

Duplicates removed (%)

81.3

Number of peaks

5198 (qval < 1E-05)

mm9

Number of total reads

164483236

Reads aligned (%)

69.8

Duplicates removed (%)

81.4

Number of peaks

5292 (qval < 1E-05)