GSM2718673: SA2 HCAEC ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
STAG2
Cell type
Cell type Class
Cardiovascular
Cell type
Coronary artery endothelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Human Coronary Artery Endothelial Cells (HCAEC)
cell type
Primary cells
experiment
ChIP-SEQ
catalogue number
Lonza CC-2585
passages
less than 10
chip antibody
custom made antibody for SA2 (STAG2)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples went through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters with "NEBNext Ultra II DNA Library Prep Kit for Illumina" from New England BioLabs (catalog # E7645). Adapter-ligated library was completed by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Median fragment size is 360 bp from which 120 bp correspond to adaptor sequences. Library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument (see below) by following manufacturer's protocols.