GSM2717253: Control P493 input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
P493-6
NA
NA
Attributes by original data submitter
Sample
source_name
P493-6 cells
cell types
B cell
genotype
scramble shRNA
treatment
Input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard ChIP assays was performed. Library preparation and sequencing for RNA and ChIP (and their corresponding input) DNA samples were performed at the Genomic Services Lab at HudsonAlpha Institute for Biotechnology (Huntsville, AL). Briefly, the quality of the total RNA and DNA was assessed using the Agilent 2100 Bioanalyzer. Two rounds of polyA+ selection was performed for RNA samples, followed by conversion to cDNAs. The mRNA library generation kits (Agilent, Santa Clara, CA) and TruSeq ChIP Sample Prep Kit (Illumina) were used generate sequencing libraries per manufacturer’s instructions. The indexed DNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems, Woburn, MA) prior to cluster generation. Clusters were generated to yield approximately 725 K to 825 K clusters/mm2. Cluster density and quality were determined during the run after the first base addition parameters were assessed.