Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

OCI-AML3

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

OCI-AML3 cells

method

ChIP-seq

chip antibody

H3K27ac (Diagenode, #C15410196)

treatment

None

replicate

rep1

internal id

J4772

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from 10 ng of ChIP material per sample. NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240L) and for the quantity measurement KAPA Library Quantification Kit (KapaBiosystems, #KK4824) was used according to the manufacturer’s instructions. 2 nM samples were multiplexed for sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

10447788

Reads aligned (%)

82.2

Duplicates removed (%)

16.2

Number of peaks

12804 (qval < 1E-05)

hg19

Number of total reads

10447788

Reads aligned (%)

81.9

Duplicates removed (%)

16.6

Number of peaks

12779 (qval < 1E-05)