GSM2716708: ChIPseq NCI-H2171 BRD4 NA NA NA NA NA NA rep1 J2386; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Lung
Cell type
NCI-H2171
Primary Tissue
Lung
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Carcinoma Small Cell
Attributes by original data submitter
Sample
source_name
NCI-H2171 cells
method
ChIP-seq
chip antibody
BRD4 (Bethyl, #A301-985A50)
treatment
None
replicate
rep1
internal id
J2386
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from 10 ng of ChIP material per sample. NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240L) and for the quantity measurement KAPA Library Quantification Kit (KapaBiosystems, #KK4824) was used according to the manufacturer’s instructions. 2 nM samples were multiplexed for sequencing.