Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV-4-11-B cells
method
ChIP-seq
chip antibody
BRD4 (Bethyl, #A301-985A50)
treatment
BI00894999, 1000nM, 4h
replicate
rep1
internal id
J3756

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from 10 ng of ChIP material per sample. NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240L) and for the quantity measurement KAPA Library Quantification Kit (KapaBiosystems, #KK4824) was used according to the manufacturer’s instructions. 2 nM samples were multiplexed for sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
144706435
Reads aligned (%)
91.6
Duplicates removed (%)
5.4
Number of peaks
1782 (qval < 1E-05)

hg38

Number of total reads
144706435
Reads aligned (%)
93.1
Duplicates removed (%)
4.4
Number of peaks
1824 (qval < 1E-05)

Base call quality data from DBCLS SRA