Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MOLM-13 cells
method
ChIP-seq
chip antibody
None
treatment
None
replicate
rep1
internal id
J4762

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared from 10 ng of ChIP material per sample. NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240L) and for the quantity measurement KAPA Library Quantification Kit (KapaBiosystems, #KK4824) was used according to the manufacturer’s instructions. 2 nM samples were multiplexed for sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg38

Number of total reads
100678526
Reads aligned (%)
97.6
Duplicates removed (%)
5.6
Number of peaks
2153 (qval < 1E-05)

hg19

Number of total reads
100678526
Reads aligned (%)
96.5
Duplicates removed (%)
7.2
Number of peaks
1572 (qval < 1E-05)

Base call quality data from DBCLS SRA