Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
mESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
ZBTB2-GFP BAC IB10 ESC-derived NPCs
strain background
129/Ola
cell type
Neural progenitor cells
genotype/variation
ZBTB2-GFP BAC IB10 ESC-derived NPCs
culture conditions
NPC medium
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer’s instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
31325008
Reads aligned (%)
94.7
Duplicates removed (%)
13.1
Number of peaks
6112 (qval < 1E-05)

mm9

Number of total reads
31325008
Reads aligned (%)
94.5
Duplicates removed (%)
14.0
Number of peaks
6036 (qval < 1E-05)

Base call quality data from DBCLS SRA