Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

ZBTB2-GFP BAC IB10 ESCs

strain background

129/Ola

cell type

Embryonic stem cells

genotype/variation

ZBTB2-GFP BAC IB10 ESCs

culture conditions

Serum/LIF + 2i

chip antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer’s instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

53201677

Reads aligned (%)

96.0

Duplicates removed (%)

14.9

Number of peaks

743 (qval < 1E-05)

mm9

Number of total reads

53201677

Reads aligned (%)

95.7

Duplicates removed (%)

14.8

Number of peaks

893 (qval < 1E-05)