Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
ME-1
Primary Tissue
Bone Marrow
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
ME-1 cells
cell line
ME-1
cell type
Human inv(16) leukemia cell line
treated with
AI-10-49
chip antibody
Anti-RUNX1/AML1 antibody
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and RUNX1-DNA and histone-DNA complexes were isolated with antibodies. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
43473708
Reads aligned (%)
83.7
Duplicates removed (%)
13.0
Number of peaks
8828 (qval < 1E-05)

hg38

Number of total reads
43473708
Reads aligned (%)
85.8
Duplicates removed (%)
11.6
Number of peaks
8839 (qval < 1E-05)

Base call quality data from DBCLS SRA