Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
ME-1
Primary Tissue
Bone Marrow
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
ME-1 cells
cell line
ME-1
cell type
Human inv(16) leukemia cell line
treated with
AI-10-49
chip antibody
Anti-Histone H3 (acetyl K27) antibody
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and RUNX1-DNA and histone-DNA complexes were isolated with antibodies. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
49068928
Reads aligned (%)
99.0
Duplicates removed (%)
13.3
Number of peaks
33088 (qval < 1E-05)

hg19

Number of total reads
49068928
Reads aligned (%)
98.5
Duplicates removed (%)
13.9
Number of peaks
33127 (qval < 1E-05)

Base call quality data from DBCLS SRA