Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Spleen CD4+ T cells
cell type
Naives T lymphocytes
number of culture days
0
strain
C57BL/6 Setdb1+/+
antibody
H3K9me3 (ab8898 from Abcam; lot GR114265-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-seq: Total RNA was extracted by using the RNeasy Micro Kit (Qiagen). For ChIP-seq: DNA purification was performed by column (MiniElute PCR purification kit, Qiagen). Libraries were prepared for sequencing using standard TruSeq Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 3000

mm10

Number of total reads
48809202
Reads aligned (%)
93.7
Duplicates removed (%)
34.6
Number of peaks
296 (qval < 1E-05)

mm9

Number of total reads
48809202
Reads aligned (%)
93.6
Duplicates removed (%)
34.8
Number of peaks
258 (qval < 1E-05)

Base call quality data from DBCLS SRA