Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

Emrbyonic neural stem cells

strain

CD-1

age

E14.5

cell type

Embryonic neural stem cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslink was performed with disuccinimidyl glutarate (DSG) (Thermo Scientifis) for 45 minutes followed by 30 minutes incubation with formaldehyde 1%. The reaction was blocked with glycine 100 mM. Shearing was performed using Covaris S2 (Covaris, Woburn, MA) for 8 min at maximum intensity. The sonicated chromatin was incubated O/N at 4°C in presence of 5µg of anti-Foxp1 (ab16645, Abcam) coupled to A/G sepharose beads (Santa Cruz Biotechnology). Kapa Hyper Prep Kit (Kapa Biosystems, Wilmington, MA) was used for End-repair, A-tailing and ligation of sequence adaptors. Samples were amplified by PCR and the libraries were size-selected in the 200-500 bp range.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

2617523

Reads aligned (%)

86.5

Duplicates removed (%)

6.2

Number of peaks

32 (qval < 1E-05)

mm9

Number of total reads

2617523

Reads aligned (%)

86.2

Duplicates removed (%)

6.4

Number of peaks

30 (qval < 1E-05)