Activated CD4+ T cell under IL6+TGFbR inhibitor condition
background
C57BL/6
gender
female
tissue
Spleen+Lymph nodes
cell type
CD4+T cell
timepoint
12 hour post treatment
treatment
IL6+TGFbR inhibitor
genotype
WT
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested and fixed with FA for 20min at 37C. And then performed ChIP. Libraries were constructed with the use of the Illumina TruSeq DNA library construction kit V2 according to manufacturer's protocol. Libaries were pooled and sequenced on the Illumina NextSeq500 sequencer