ChIP analyses in 3T3-L1 and C3H10T1/2 cells were performed as described previously (Schupp, M. et al. Retinol saturase promotes adipogenesis and is downregulated in obesity. Proc. Natl. Acad. Sci. U.S.A. 106, 1105–1110 (2009)). Approx. 100 µg of sonicated chromatin extracts were used for each IP and incubated with 10 µg of a GATA2 antibody (sc-9008x, Santa Cruz) overnight. For deep sequencing ChIP samples (100-150ng of pooled DNA derived from individual CHIP experiments) were first amplified using the ChIP-Seq Sample Prep Kit (Illumina) according to the manufacturers instructions.