Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Uterus

Cell type

Cervical cancer cells

NA

NA

Sample

source_name

cervical cancer cells

genotype/variation

vector

chip antibody

H3K4me1(ABclonal, A2355)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Clarified lysates were diluted with ChIP dilution buffer,precleared with salmon sperm DNA blocked Protein G Sepharose 4 Fast Flow for 1 h at 4°c and incubated overnight at 4°c with primary antibodies respectively. Protein-DNA complex is then precipitated with salmon sperm DNA blocked Protein G Sepharose beads at 4°c for 4 h. Bound complexes were washed once .ChIP DNA were prepared from the overnight samples. Total RNA from cells were extracted using TRIzol reagent following the manufacturer's instructions. Sequencing of samples was performed on HiSeq 2500 (Illumina, CA, USA) platform according to Illumina’s protocol.Directional RNA Library Prep Kit for Illumina® (NEB, USA)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31669679

Reads aligned (%)

84.9

Duplicates removed (%)

14.7

Number of peaks

18943 (qval < 1E-05)

hg19

Number of total reads

31669679

Reads aligned (%)

84.6

Duplicates removed (%)

15.3

Number of peaks

19187 (qval < 1E-05)