GSM2706140: KDM5C Input ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
Cervical cancer cells
NA
NA
Attributes by original data submitter
Sample
source_name
cervical cancer cells
genotype/variation
KDM5C overexpression
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Clarified lysates were diluted with ChIP dilution buffer,precleared with salmon sperm DNA blocked Protein G Sepharose 4 Fast Flow for 1 h at 4°c and incubated overnight at 4°c with primary antibodies respectively. Protein-DNA complex is then precipitated with salmon sperm DNA blocked Protein G Sepharose beads at 4°c for 4 h. Bound complexes were washed once .ChIP DNA were prepared from the overnight samples. Total RNA from cells were extracted using TRIzol reagent following the manufacturer's instructions. Sequencing of samples was performed on HiSeq 2500 (Illumina, CA, USA) platform according to Illumina’s protocol.Directional RNA Library Prep Kit for Illumina® (NEB, USA)