Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ubx

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2-HA::Ubx Cell line
cell line
late embryo-derived cell line stably transfected with HA::Ubx
strain
S2-DRSC-HA::Ubx
genotype
HA::Ubx
treatment
10 microMolar Cu2SO4
chip antibody
HA (AbCam ab9110)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared according to the modENCODE procedure; RNA was extracted with Qiagen Rneasy kit DNA libraries were prepared according to Illumina's instructions; RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
50285491
Reads aligned (%)
57.9
Duplicates removed (%)
34.6
Number of peaks
16786 (qval < 1E-05)

dm6

Number of total reads
50285491
Reads aligned (%)
56.3
Duplicates removed (%)
36.3
Number of peaks
16135 (qval < 1E-05)

Base call quality data from DBCLS SRA