Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
M1BP

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2-HA::AbdA Cell line
cell line
late embryo-derived cell line stably transfected with HA::AbdA
strain
S2-DRSC-HA::AbdA
genotype
HA::AbdA
treatment
10 microMolar Cu2SO4
chip antibody
M1BP (a gift from Dr. D. Gilmour - Li and Gilmour (2013) EMBO J. 32(13):1829-1841)
input dna code
D

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared according to the modENCODE procedure; RNA was extracted with Qiagen Rneasy kit DNA libraries were prepared according to Illumina's instructions; RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
16215395
Reads aligned (%)
97.1
Duplicates removed (%)
35.5
Number of peaks
8373 (qval < 1E-05)

dm6

Number of total reads
16215395
Reads aligned (%)
96.9
Duplicates removed (%)
36.4
Number of peaks
7878 (qval < 1E-05)

Base call quality data from DBCLS SRA