Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
M1BP

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 Cell line
cell line
late embryo-derived cell line
strain
S2-DRSC
genotype
wild type
treatment
10 microMolar Cu2SO4
chip antibody
M1BP (a gift from Dr. D. Gilmour - Li and Gilmour (2013) EMBO J. 32(13):1829-1841)
input dna code
B

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared according to the modENCODE procedure; RNA was extracted with Qiagen Rneasy kit DNA libraries were prepared according to Illumina's instructions; RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
16428120
Reads aligned (%)
97.0
Duplicates removed (%)
50.7
Number of peaks
9102 (qval < 1E-05)

dm6

Number of total reads
16428120
Reads aligned (%)
96.9
Duplicates removed (%)
51.5
Number of peaks
8674 (qval < 1E-05)

Base call quality data from DBCLS SRA