Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pc

Cell type

Cell type Class
Cell line
Cell type
ML-DmBG3-c2
Source
y[1] v[1] f[1] mal[F1]
Tissue Source
central nervous system
Developmental Stage
third instar larval stage

Attributes by original data submitter

Sample

source_name
Pc n-ChIP BG3-c2
cell line
BG3-c2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in CM Weber, JG Henikoff & S Henikoff, H2A.Z nucleosomes enriched over active genes are homotypic, Nat Struct Mol Bio 2010 Dec;17(12):1500-7, PMID 21057526 and SS Teves & S Henikoff Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. Genes Dev. 2011 Nov 15;25(22):2387-97. PubMed PMID: 22085965 A modified Solexa Illumina library construction protocol was used as previously described in SS Teves & S Henikoff Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. Genes Dev. 2011 Nov 15;25(22):2387-97. PubMed PMID: 22085965

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
27488204
Reads aligned (%)
88.6
Duplicates removed (%)
15.6
Number of peaks
2213 (qval < 1E-05)

dm6

Number of total reads
27488204
Reads aligned (%)
87.9
Duplicates removed (%)
19.0
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA