GSM2702222: ChIP SKOV-3 H3K27ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Gonad
Cell type
SK-OV-3
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
ovarian cancer cell line
cell line
SKOV-3
tissue
ovarian
antibody
H3K27ac (ab4729, abcam)
used medium
McCoy’s 5a minimal essential medium supplemented with FBS
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
A total of 20 million cells per sample were collected, yielding ~20 fractions of chromatin. Chromatin was extracted using the MagnaChIP-seq kit (millipore).Sonication was performed for 12 cycles, 30 sec pulses using the biorupter Anti-histone H3 acetyl K27 antibody (ab4729, Abcam) antibody was used for ChIP. Per sample, 5–30 ng of precipitated DNA or input was used to perform library preparation according to the Illumina TruSeq DNA Sample preparation guide. In brief, the immunoprecipitated DNA was end-repaired, A-tailed and ligated to diluted sequencing adapters (1/100). After PCR amplification (15–18 cycles) and bead purification (Agencourt AmpureXp, Analis), the libraries were sequenced using the HiSeq 2500 (Illumina).