Mice treated withNaCl only, Input control, ie no IP
tissue
human breast cancer cells from PDX mice
replicate number
first replicate
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tumors were frozen in liquid nitrogen. For ChIP the detailed protocol from Scmidt D. et al (Methods 2009) were followed, ie samples were pulverised and x-linked in PBS with 0,5% folmaldehyde for 10 minutes, then proceeded with Lysis buffers. Library construction: ThruPLEX (Rubicon Genomics, Ann Arbor, MI, USA) supplied by Diagenode. The manufacturer’s instructions were followed.