Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
bone marrow-derived macrophages
strain
C57BL/6
tissue
bone marrow
cell type
bone marrow-derived macrophages
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The chromatin was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against EZH2 ( Active Motif 39901). Libraries were prepared according to standard Illumina protocols.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
39225752
Reads aligned (%)
97.9
Duplicates removed (%)
13.4
Number of peaks
638 (qval < 1E-05)

mm9

Number of total reads
39225752
Reads aligned (%)
97.8
Duplicates removed (%)
13.4
Number of peaks
620 (qval < 1E-05)

Base call quality data from DBCLS SRA