Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
Hematopoietic Stem Cells
MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by original data submitter

Sample

source_name
Lin- BM progenitors (HSPCs)
cell type
Lin- BM progenitors (HSPCs)
genotype
UTX WT
strain
C57BL/6
chip antibody
PU.1 (Santa Cruz Biotechnology, #sc-352)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HSPCs was extracted from bone marrow using the Lineage Cell Depletion Kit (Miltenyi Biotec, cat. no. 130-090-858). Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
33533075
Reads aligned (%)
97.9
Duplicates removed (%)
12.1
Number of peaks
103439 (qval < 1E-05)

mm9

Number of total reads
33533075
Reads aligned (%)
97.8
Duplicates removed (%)
12.3
Number of peaks
103358 (qval < 1E-05)

Base call quality data from DBCLS SRA