Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Kidney
Cell type
Renal tubular cells
NA
NA

Attributes by original data submitter

Sample

source_name
Renal tubular cells
cell type
renal tubular cells
passages
4
antibody
Cayman HIF-1a (Cay10006421)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP samples were prepared according to standard ChIP procedures (Millipore protocol) and D N A was isolated using a phenol/chloroform extraction. ChIP-seq and FAIRE-seq library preparations were carried out using Illumina protocols and libraries were sequenced on HiSeq 2000/2500 platforms (Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
139463003
Reads aligned (%)
29.5
Duplicates removed (%)
67.1
Number of peaks
2732 (qval < 1E-05)

hg38

Number of total reads
139463003
Reads aligned (%)
31.3
Duplicates removed (%)
65.4
Number of peaks
4058 (qval < 1E-05)

Base call quality data from DBCLS SRA