Antigen

Antigen Class

TFs and others

Antigen

HIF1A

Cell type

Cell type Class

Kidney

Cell type

Renal tubular cells

NA

NA

Sample

source_name

Renal tubular cells

cell type

renal tubular cells

passages

4

antibody

Cayman HIF-1a (Cay10006421)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP samples were prepared according to standard ChIP procedures (Millipore protocol) and D N A was isolated using a phenol/chloroform extraction. ChIP-seq and FAIRE-seq library preparations were carried out using Illumina protocols and libraries were sequenced on HiSeq 2000/2500 platforms (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

139463003

Reads aligned (%)

90.7

Duplicates removed (%)

65.4

Number of peaks

4058 (qval < 1E-05)

hg19

Number of total reads

139463003

Reads aligned (%)

89.7

Duplicates removed (%)

67.1

Number of peaks

2732 (qval < 1E-05)