Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
RAW 264.7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Macrophages
cell type
RAW 264.7 Macrophages
genotype
wild type
strain
BALB/c
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with specific antibody. DNA libraries were prepared for HiSeq2000 sequencing as previously described (Ostuni, 2013).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50142593
Reads aligned (%)
95.2
Duplicates removed (%)
16.6
Number of peaks
596 (qval < 1E-05)

mm9

Number of total reads
50142593
Reads aligned (%)
94.8
Duplicates removed (%)
16.5
Number of peaks
681 (qval < 1E-05)

Base call quality data from DBCLS SRA