GSM2693326: Hec1A TH63, FoxA1 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXA1
Cell type
Cell type Class
Uterus
Cell type
HEC-1-A
Primary Tissue
Uterus
Site of Extraction
Endometrium
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Hec1A cell line, FOXA1-CHIP
cell line
Hec1A
chip antibody
FoxA1 rabbit ab23738 (lot GR292351-2)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries from ChIP samples were made with two different kits. With Illumina (San Diego, CA) TruSeq™ DNA Sample Preparation this modifications were used: ligation with of 1/10th the manufacturers recommended adapter amounts and agarose gel selection of DNA fragments in the 200-500 bp size range. For ThruPLEX (Rubicon Genomics, Ann Arbor, MI, USA. Supplied by Diagenode) library preparatiopn the manufacturer’s instructions were followed. ThruPLEX, Rubicon Genomics