5 days to NPCs using NB27 and Neuro2 supplements (GFP facs sorted)
cell type
NPC
chip antibody
MNase digested input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols