Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
MNase digested input DNA_embryonic stem cells
cell line
46c ESC (sox1-GFP)
differentiation
undifferentiated
cell type
ESC
chip antibody
MNase digested input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
160652470
Reads aligned (%)
94.3
Duplicates removed (%)
19.0
Number of peaks
420 (qval < 1E-05)

mm9

Number of total reads
160652470
Reads aligned (%)
94.2
Duplicates removed (%)
19.0
Number of peaks
423 (qval < 1E-05)

Base call quality data from DBCLS SRA