Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
Myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
MA9-transformed mouse leukemia
tissue
bone marrow
cell markers
Lin-Sca-1+c-Kit+
genotype
MLL-AF9 fusion
chip antibody
anti-PU.1 (Santa Cruz, sc-352)
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
58042045
Reads aligned (%)
93.6
Duplicates removed (%)
16.9
Number of peaks
80182 (qval < 1E-05)

mm9

Number of total reads
58042045
Reads aligned (%)
93.3
Duplicates removed (%)
16.8
Number of peaks
80232 (qval < 1E-05)

Base call quality data from DBCLS SRA