Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kdm1a

Cell type

Cell type Class
Blood
Cell type
Myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
MA9-transformed mouse leukemia
tissue
bone marrow
cell markers
Lin-Sca-1+c-Kit+
genotype
MLL-AF9 fusion
chip antibody
anti-LSD1 (Abcam, ab17721)
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
28578237
Reads aligned (%)
94.8
Duplicates removed (%)
16.1
Number of peaks
609 (qval < 1E-05)

mm9

Number of total reads
28578237
Reads aligned (%)
94.4
Duplicates removed (%)
16.0
Number of peaks
659 (qval < 1E-05)

Base call quality data from DBCLS SRA