Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Cebpa

Cell type

Cell type Class
Blood
Cell type
Myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
MA9-transformed mouse leukemia
tissue
bone marrow
cell markers
Lin-Sca-1+c-Kit+
genotype
MLL-AF9 fusion
chip antibody
anti-Cebpa (Santa Cruz, sc-61)
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
14099116
Reads aligned (%)
92.9
Duplicates removed (%)
23.0
Number of peaks
16078 (qval < 1E-05)

mm9

Number of total reads
14099116
Reads aligned (%)
92.5
Duplicates removed (%)
23.1
Number of peaks
16115 (qval < 1E-05)

Base call quality data from DBCLS SRA