Antigen

Antigen Class

TFs and others

Antigen

Pitx1

Cell type

Cell type Class

Embryo

Cell type

Hindlimb

MeSH Description

Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)

Sample

source_name

e11.5 HL

developmental stage

e11.5

tissue

hindlimb

genotype

wild type

strain

CD1

chip antibody

homemade (960A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Tissue was fixed with 1% formaldehyde, nuclei were isolated, genomic DNA was sheared by sonication, and protein:DNA complexes were pulled down with the indicated antibodies. Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

61819460

Reads aligned (%)

97.0

Duplicates removed (%)

16.0

Number of peaks

18833 (qval < 1E-05)

mm9

Number of total reads

61819460

Reads aligned (%)

96.9

Duplicates removed (%)

16.7

Number of peaks

18828 (qval < 1E-05)