Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
Hindlimb
MeSH Description
Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)

Attributes by original data submitter

Sample

source_name
e11.0 HL
developmental stage
e11.0
tissue
hindlimb
genotype
wild type
strain
CD1
chip antibody
CST #9751BF

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was fixed with 1% formaldehyde, nuclei were isolated, genomic DNA was sheared by sonication, and protein:DNA complexes were pulled down with the indicated antibodies. Illumina

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
54437068
Reads aligned (%)
97.4
Duplicates removed (%)
25.6
Number of peaks
24575 (qval < 1E-05)

mm9

Number of total reads
54437068
Reads aligned (%)
97.2
Duplicates removed (%)
25.6
Number of peaks
24614 (qval < 1E-05)

Base call quality data from DBCLS SRA