Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Forelimb
MeSH Description
A front limb of a quadruped. (The Random House College Dictionary, 1980)

Attributes by original data submitter

Sample

source_name
e10.5 FL
developmental stage
e10.5
tissue
forelimb
genotype
wild type
strain
CD1
chip antibody
CST #9733BC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was fixed with 1% formaldehyde, nuclei were isolated, genomic DNA was sheared by sonication, and protein:DNA complexes were pulled down with the indicated antibodies. Illumina

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
50966427
Reads aligned (%)
97.3
Duplicates removed (%)
11.6
Number of peaks
16585 (qval < 1E-05)

mm9

Number of total reads
50966427
Reads aligned (%)
97.2
Duplicates removed (%)
11.6
Number of peaks
16604 (qval < 1E-05)

Base call quality data from DBCLS SRA