Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Embryo
Cell type
Early embryo
NA
NA

Attributes by original data submitter

Sample

source_name
early embryo
strain
set-25(n5021) III
tissue
early embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were fixed with 2% Formalin for 30min at room temperature. Fixed chromatin was extracted using the FastPrep-24™ (MPI) homogenizer and sonicated using the Bioruptor (Diagenode) Libraries were prepared from chromatin IP and input samples using the NEBNext ultra DNA library prep kit for Illumina (NEB # 7370) and the NEBNext Multiplex Oligos for Illumina (NEB # E7335)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
17969658
Reads aligned (%)
96.6
Duplicates removed (%)
35.8
Number of peaks
6192 (qval < 1E-05)

ce10

Number of total reads
17969658
Reads aligned (%)
96.6
Duplicates removed (%)
35.8
Number of peaks
6192 (qval < 1E-05)

Base call quality data from DBCLS SRA