Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
POU5F1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells mixed with fibroblast cells
cell type
1%H1 ESCs mixed with 99% fibroblast
antibody
anti-OCT4 (sc-8628, Santa Cruz Biotechnology);

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed with 1% SDS Lysis Buffer (on ice, 5 min) and then centrifuged (2,000 rpm, 10 min). Remove supernatant as described above and resuspend samples in 0.1% SDS buffer with PIC (1ml 0.1%SDS buffer for every 100ul nuclear pellet). Samples were then sonicated with glass beads for 12 times (30 s pulses with 30 s break interval) using the Bioruptor water bath sonicator (Diagenode). Libraries were prepared according to Illumina's instructions by Stanford genomic core facility

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
23531576
Reads aligned (%)
24.6
Duplicates removed (%)
67.6
Number of peaks
1357 (qval < 1E-05)

hg38

Number of total reads
23531576
Reads aligned (%)
25.5
Duplicates removed (%)
66.7
Number of peaks
1124 (qval < 1E-05)

Base call quality data from DBCLS SRA