Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Cerebral Cortex
MeSH Description
Type of cerebral cortex which does not pass through a perinatal phase of six-layered structure as in the NEOCORTEX and develops into three or four layers in the mature brain. Allocortex has three subareas: archi- paleo- and periallo-cortex.

Attributes by original data submitter

Sample

source_name
cerebral cortex
strain
C57BL/6
genotype
Rosa26 CAG-floxed-pA-H2B-FLAG/ CaMKCreERT2
chip antibody
None
tissue
cerebral cortex
replicate
Replicate3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated DNAs complexed with histone were isolated with antibodies. Libraries were prepared by KAPA LTP Library Preparation Kit (KAPA Biosystems) with TruSeq index adaptors, according to manufacture's instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
12861790
Reads aligned (%)
99.0
Duplicates removed (%)
10.4
Number of peaks
286 (qval < 1E-05)

mm9

Number of total reads
12861790
Reads aligned (%)
98.9
Duplicates removed (%)
10.5
Number of peaks
235 (qval < 1E-05)

Base call quality data from DBCLS SRA