Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Larvae

Cell type

L4

NA

NA

Sample

source_name

set-9set-26_whole_worm

genotype

set-9 set-26

developmental stage

L4

chip antibody

anti-H3 (abcam, ab1791, lot:GR135488-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked protein-DNA complex were isolated with antibody. ChIP-seqw libraries were prepared for sequencing using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

ce11

Number of total reads

25229792

Reads aligned (%)

91.3

Duplicates removed (%)

11.4

Number of peaks

549 (qval < 1E-05)

ce10

Number of total reads

25229792

Reads aligned (%)

91.3

Duplicates removed (%)

11.4

Number of peaks

551 (qval < 1E-05)